Overview
Description
Theory and practical aspects of microscope-based techniques currently utilized in research in both the life and physical sciences.
Units
Lecture3
Catalog Details
Offering
Offered: Other
Related Courses
Cross Listed With BME 730
Attributes
Standard
Learning Outcomes
- demonstrate understanding of the physical properties of light and how it influences the optical properties and limit of systems used to image biological samples.
- demonstrate how to prepare biological samples for histology, immunocytochemistry and immunohistochemistry.
- demonstrate familiarity with optics, advanced microscopy techniques such as visible light, confocal, total internal reflection, multiphoton and super resolution fluorescence microscopy, atomic force microscopy and imaging.
- recognize the principles underlying advanced spectral analysis of protein-protein interactions and co-localization, and molecular diffusion by Förster (FRET) and Bioluminescence (BRET) Resonance Energy Transfer, Fluorescence Recovery After Photobleaching (FRAP), Fluorescence Lifetime Imaging Microscopy (FLIM) and Fluorescence Correlation Spectroscopy (FCS).
- recognize the principles and applications of non-invasive in vivo imaging by ultrasonography and Doppler imaging, and nuclear magnetic resonance imaging.